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. 2010 Dec;76(24):8269-76.
doi: 10.1128/AEM.00687-10. Epub 2010 Oct 22.

Multiple unique Cryptosporidium isolates from three species of ground squirrels (Spermophilus beecheyi, S. beldingi, and S. lateralis) in California

Maria das Graças Cabral Pereira  1 , Xunde Li, Brenda McCowan, Ralph L Phillips, Edward R Atwill
Affiliations

Multiple unique Cryptosporidium isolates from three species of ground squirrels (Spermophilus beecheyi, S. beldingi, and S. lateralis) in California

Maria das Graças Cabral Pereira et al. Appl Environ Microbiol. 2010 Dec.

Abstract

Phylogenetic analyses of the 18S small-subunit (SSU) rRNA and Cryptosporidium oocyst wall protein (COWP) genes indicate that multiple unique Cryptosporidium isolates are shed by separate species of Spermophilus ground squirrels (S. beecheyi, S. beldingi, and S. lateralis) throughout California, despite some squirrel populations' being geographically isolated. The data support our assertion that Spermophilus squirrels shed novel Cryptosporidium species.

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Figures

FIG. 1.
FIG. 1.
Geographic locations of three different species of Spermophilus ground squirrels from which 49 isolates of Cryptosporidium were collected in California from 2000 to 2004.
FIG. 2.
FIG. 2.
Phylogenetic relationships of partial (∼750-bp) 18S SSU rRNA sequences from Cryptosporidium species, genotypes, and novel Cryptosporidium isolates from three species of Spermophilus ground squirrels, S. beecheyi, S. lateralis, and S. beldingi, inferred by neighbor-joining analyses with 1,000 bootstrap replicates.
FIG. 3.
FIG. 3.
Phylogenetic relationships of the entire 18S SSU rRNA sequences (∼1,750 bp) from Cryptosporidium species, genotypes, and novel Cryptosporidium isolates from three species of Spermophilus ground squirrels, S. beecheyi, S. lateralis, and S. beldingi, inferred by neighbor-joining analyses with 1,000 bootstrap replicates.
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