Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011:691:271-9.
doi: 10.1007/978-1-60761-849-2_16.

Evaluation of embryotoxicity using the zebrafish model

Lisa Truong  1 , Stacey L Harper, Robert L Tanguay
Affiliations

Evaluation of embryotoxicity using the zebrafish model

Lisa Truong et al. Methods Mol Biol. 2011.

Abstract

The embryonic zebrafish model offers the power of whole-animal investigations (e.g., intact organism, functional homeostatic feedback mechanisms, and intercellular signaling) with the convenience of cell culture (e.g., cost- and time-efficient, minimal infrastructure, small quantities of nanomaterial solutions required). The model system overcomes many of the current limitations in rapid to high-throughput screening of drugs/compounds and casts a broad net to evaluate integrated system effects rapidly. Additionally, it is an ideal platform to follow up with targeted studies aimed at the mechanisms of toxic action. Exposures are carried out in 96-well plates so minimal solution volumes are required for the assessments. Numerous morphological, developmental, and behavioral endpoints can be evaluated noninvasively due to the transparent nature of the embryos.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1. Six hours post fertilization embryos
a) Six hpf embryo with its chorion. b) Six hpf embryo after using pronase to enzymatically remove its chorion.
Fig. 2
Fig. 2. Visual assessment of zebrafish morphology
Images are given as examples of typical chemical-induced malformations observed in the zebrafish.
See this image and copyright information in PMC

References

    1. Akimenko MA, Johnson SL, et al. Differential induction of four msx homeobox genes during fin development and regeneration in zebrafish. Development. 1995;121(2):347–57. - PubMed
    1. Aparicio S, Chapman J, et al. Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes. Science. 2002;297(5585):1301–10. - PubMed
    1. Blechinger SR, Warren JT, Jr, et al. Developmental toxicology of cadmium in living embryos of a stable transgenic zebrafish line. Environ Health Perspect. 2002;110(10):1041–6. - PMC - PubMed
    1. Busquet F, Nagel R, et al. Development of a new screening assay to identify proteratogenic substances using zebrafish danio rerio embryo combined with an exogenous mammalian metabolic activation system (mDarT) Toxicol Sci. 2008;104(1):177–88. - PubMed
    1. Harper SL, Dahl JL, et al. Proactively designing nanomaterials to enhance performance and minimize hazard. International Journal of Nanotechnology. 2008;5(1):124–142.

Publication types

LinkOut - more resources