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. 2011 Jan 12:1368:102-7.
doi: 10.1016/j.brainres.2010.10.079. Epub 2010 Oct 25.

The acute antinociceptive effect of HBO₂ is mediated by a NO-cyclic GMP-PKG-KATP channel pathway in mice

Lindsay P Quock  1 , Yao Zhang, Eunhee Chung, Yusuke Ohgami, Donald Y Shirachi, Raymond M Quock
Affiliations

The acute antinociceptive effect of HBO₂ is mediated by a NO-cyclic GMP-PKG-KATP channel pathway in mice

Lindsay P Quock et al. Brain Res. .

Abstract

Previous research has found that hyperbaric oxygen (HBO(2)) produces an acute antinociceptive effect that is dependent on nitric oxide (NO). The present study was undertaken to determine whether HBO(2)-induced acute antinociception might involve a NO-cyclic GMP-protein kinase G-ATP-sensitive potassium (K(ATP)) channel pathway. Male NIH Swiss mice were subjected to a 5-min HBO(2) treatment (100% oxygen at 3.5 absolute atmospheres) and antinociception was assessed over the next 6 min still under HBO(2) using the acetic acid abdominal constriction test. Pretreatment with 2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazolyl-1-oxy-3-oxide (carboxy-PTIO, an NO scavenger), 1H-[1,2,4]-oxadiazolo-[4,3-a]quinoxalin-1-one) (a soluble guanylyl cyclase-inhibitor, Rp-8-(4-chlorophenylthio)-guanosine-3',5'-cyclic monophosphorothioate (a protein kinase G-inhibitor) or glibenclamide (an ATP-sensitive potassium channel-inhibitor) all led to antagonism of the HBO(2)-induced acute antinociception in a dose-dependent manner. These findings suggest that HBO(2)-induced acute antinociception might be due to activation of a NO-cyclic GMP-protein kinase G-K(ATP) channel pathway.

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Figures

Fig. 1
Fig. 1
Effect of carboxy-PTIO on the antinociceptive effect of HBO2 at 3.5 ATA. Each bar represents the mean antinociceptive effect and each vertical line represents the SEM of at least 8 mice per group. Significance of difference: ***, P < 0.001, compared to the vehicle pretreatment group (post-hoc Bonferroni's multiple comparison test).
Fig. 2
Fig. 2
Effect of ODQ on the antinociceptive effect of HBO2 at 3.5 ATA. Each bar represents the mean antinociceptive effect and each vertical line represents the SEM of at least 8 mice per group. Significance of difference: ***, P < 0.001, compared to the vehicle pretreatment group (post-hoc Bonferroni's multiple comparison test).
Fig. 3
Fig. 3
Effect of Rp-8-pCPT-cGMPS on the antinociceptive effect of HBO2 at 3.5 ATA. Each bar represents the mean antinociceptive effect and each vertical line represents the SEM of at least 8 mice per group. Significance of difference: ***, P < 0.001, compared to the vehicle pretreatment group (post-hoc Bonferroni's multiple comparison test).
Fig. 4
Fig. 4
Effect of glibenclamide on the antinociceptive effect of HBO2 at 3.5 ATA. Each bar represents the mean antinociceptive effect and each vertical line represents the SEM of at least 8 mice per group. Significance of difference: ***, P < 0.001, compared to the vehicle pretreatment group (post-hoc Bonferroni's multiple comparison test).
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