Two-dimensional electrophoresis of proteins: a simple procedure for casting of narrow-bore, first dimension (isoelectric focusing) polyacrylamide tube gels
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- PMID: 2098102
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Two-dimensional electrophoresis of proteins: a simple procedure for casting of narrow-bore, first dimension (isoelectric focusing) polyacrylamide tube gels
Appl Theor Electrophor.
1990.
Abstract
A convenient and rapid method for casting of narrow-bore (1.5-2.5 mm, i.d.) first dimension isoelectric focusing gel tubes is described. The procedure utilizes a peristaltic pump and a 2-inch, 23 gauge blunt needle for dispensing the gel into fixed tubes on a 1.1 degree incline. The method is simple to perform, precludes the formation of air bubbles and little or no manual dexterity is required.
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