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. 2011:2011:904532.
doi: 10.1155/2011/904532. Epub 2010 Oct 14.

Inhibitory Effects of Terminalia catappa on UVB-Induced Photodamage in Fibroblast Cell Line

Kuo-Ching Wen  1 , I-Chen Shih, Jhe-Cyuan Hu, Sue-Tsai Liao, Tsung-Wei Su, Hsiu-Mei Chiang
Affiliations

Inhibitory Effects of Terminalia catappa on UVB-Induced Photodamage in Fibroblast Cell Line

Kuo-Ching Wen et al. Evid Based Complement Alternat Med. 2011.

Abstract

This study investigated whether Terminalia catappa L. hydrophilic extract (TCLW) prevents photoaging in human dermal fibroblasts after exposure to UVB radiation. TCLW exhibited DPPH free radical scavenging activity and protected erythrocytes against AAPH-induced hemolysis. In the gelatin digestion assay, the rates of collagenase inhibition by TCL methanol extract, TCLW, and its hydrolysates were greater than 100% at the concentration of 1 mg/mL. We found that serial dilutions of TCLW (10-500 μg/mL) inhibited collagenase activity in a dose-dependent manner (82.3% to 101.0%). However, TCLW did not significantly inhibit elastase activity. In addition, TCLW inhibited MMP-1 and MMP-9 protein expression at a concentration of 25 μg/mL and inhibited MMP-3 protein expression at a concentration of 50 μg/mL. TCLW also promoted the protein expression of type I procollagen. We also found that TCLW attenuated the expression of MMP-1, -3, and -9 by inhibiting the phosphorylation of ERK, JNK, and p38. These findings suggest that TCLW increases the production of type I procollagen by inhibiting the activity of MMP-1, -3 and -9, and, therefore, has potential use in anti-aging cosmetics.

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Figures

Figure 1
Figure 1
DPPH radical scavenging activity of TCLW. The TCLW provided a strong inhibitory effect and in a dose-dependent manner (25–1000 μg/mL) on DPPH scavenging. (n = 4; *P < .05; **P < .01; ***P < .001).
Figure 2
Figure 2
The time course inhibition of TCLW on AAPH-induced lysis of rat erythrocyte. The TCLW provided a strong inhibitory effect and in a dose-dependent manner (50–500 μg/mL) against erythrocyte hemolysis.
Figure 3
Figure 3
The inhibition of Terminalia catappa L. water extract, methanol extract, and its hydrolysates on collagenase activity. (a) Terminalia catappa L. water extract (TCLW), and methanol extract (TCLM), (b) Terminalia catappa L. water extract (TCLW) and its hydrolysates 1 mg/mL, and (c) Terminalia catappa L. water extract (TCLW) 0.05–1 mg/mL (H1: hydrolyzed by 0.6 N HCl for 30 min; H2: 1.2 N HCl for 30 min; H3: hydrolyzed by 0.6 N HCl for 60 min; H4: 1.2 N HCl for 60 min) (positive control, DC 100: doxycycline 100 μg/mL; control: dd water) (n = 4; *P < .05; **P < .01; ***P < .001).
Figure 4
Figure 4
The inhibition rate (%) of TCLW on bacterial collagenase activity using fluorometric assay. (n = 4; **P < .01).
Figure 5
Figure 5
The inhibition rate of TCLW on porcine elastase. (n = 4).
Figure 6
Figure 6
Effect of TCLW on the UVB-induced expression of MMP-1, 3, 9, and type I procollagen in human fibroblasts. (n = 4; *P < .05; **P < .01; ***P < .001).
Figure 7
Figure 7
Effect of TCLW on the UVB-induced expression of MAP kinases in human fibroblasts. (n = 4; *P < .05; **P < .01).
Figure 8
Figure 8
Cell viability of TCLW on human fibroblasts. (n = 4; **P < .01; ***P < .001).
Figure 9
Figure 9
The mechanisms of TCLW on UVB-induced photodamage.
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