Chrysoeriol isolated from Eurya cilliata leaves protects MC3T3-E1 cells against hydrogen peroxide-induced inhibition of osteoblastic differentiation

Abstract

Chrysoeriol is a flavonoid compound found in several tropical medicinal plants. To elucidate the protective effects of chrysoeriol isolated from Eurya cilliata on the response of osteoblasts to oxidative stress, osteoblastic MC3T3-E1 cells were incubated with chrysoeriol and/or H₂O₂, and markers of osteoblast function and oxidative damage were examined. Chrysoeriol treatment significantly (P < 0.05) reversed the cytotoxic effect of H₂O₂ and increased collagen content, alkaline phosphatase activity and calcium deposition of osteoblasts in the presence of H₂O₂. These effects were blocked by ICI182780, suggesting that chrysoeriol's effect might be partly involved in estrogen action. Moreover, H₂O₂-induced reduction of osteocalcin was recovered in the presence of chrysoeriol. Chrysoeriol significantly (P < 0.05) decreased the production of receptor activator of nuclear factor-κB ligand, interleukin-6, protein carbonyl and malondialdehyde of MC3T3-E1 cells in the presence of H₂O₂. These results demonstrate that chrysoeriol isolated from E. cilliata can protect osteoblasts from oxidative stress-induced toxicity.