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Comparative Study
. 1990;31(4):413-22.
doi: 10.1186/BF03547523.

Evaluation of ELISA for the detection of Toxoplasma antibodies in swine sera

V Hirvelä-Koski  1
Affiliations
Comparative Study

Evaluation of ELISA for the detection of Toxoplasma antibodies in swine sera

V Hirvelä-Koski. Acta Vet Scand. 1990.

Abstract
in English, Swedish

Enzyme-linked immunosorbent assay (ELISA) is compared with the indirect fluorescent antibody test (IFAT), the indirect haemagglutination test (IHAT) and the latex agglutination (LA) test for the detection of toxoplasma antibodies in swine sera. The 100 swine sera examined represent ELISA values from greater than 0 to 154 EIU. The agreement was highest (0.67) between ELISA and IFAT with an ELISA cut-off value of 30 EIU, and between ELISA and the LA test with an ELISA cut-off value of 50 EIU (0.74). All sera giving less than 10 EIU were negative in the other tests, and all those with greater than 70 EIU were positive in 1, 2 or all of the reference tests. In order to avoid false positive results with ELISA, all sera giving 10-70 EIU should be confirmed with a test which has a good specificity, e.g. IFAT. ELISA is a sensitive test and is highly suitable for the screening of large amounts of samples, but it may be too complicated for screening toxoplasma antibodies in the laboratories of abattoirs.

ELISA (enzyme-linked immunosorbent assay) jämförs med IFAT (indirect fluorescent antibody test), IHAT (indirect haemagglutination test) och LA (latex agglutination) test for att mäta antikroppar mot Toxoplasma gondii i sera av 100 svin. Alla sera med < 10 EIU var negativa med andra tester och alla med > 70 EIU var positiva med 1, 2 eller 3 av de andra testerna. Hälften av sera med 10–70 EIU var positiva med andra tester och hälften var negativa. Det bästa “cut-off” värdet för ELISA var 30 EIU jämfört med IFAT och 50 EIU jämfört med LA test. Om man vill undvika fel positiva resultat med ELISA, borde alla sera med 10–70 EIU testas med t. ex. IFAT.

ELISA är sensitiv och lämplig för undersökning av stora mängder prov. Det kan ändå vara tekniskt för svårt att utföra på slakterilaboratorier, vårs kapacitet är inriktad mest på bakteriologiska och sensoriska undersökningar.

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References

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