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. 1990 Jan;14(1):41-50.
doi: 10.1007/BF00015653.

Expression of a rice glutelin promoter in transgenic tobacco

D J Leisy  1 , J Hnilo, Y Zhao, T W Okita
Affiliations

Expression of a rice glutelin promoter in transgenic tobacco

D J Leisy et al. Plant Mol Biol. 1990 Jan.

Abstract

A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days after flowering and obtained a maximum level at 16 days after flowering, the onset of overall protein accumulation. Significant expression of CAT activity in non-seed tissues occurred in some, but not all plants, suggesting possible chromosome position effects on non-seed tissue expression. A positive correlation was observed between expression levels in seeds and gene copy numbers.

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References

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