Constraction of a subtracted cDNA library using oligo(dT)-latex
Affiliations
- PMID: 2101904
Item in Clipboard
Constraction of a subtracted cDNA library using oligo(dT)-latex
Nucleic Acids Symp Ser.
1990.
Abstract
An efficient method for constraction of subtracted cDNA library was developed using oligo(dT 30. Latex and PCR. This method improved the chances for identifying cDNA clones corresponding to scarce class of mRNA that is expressed differentially during cellular growth and differentiation.
Similar articles
-
DNA-DNA subtractive cDNA cloning using oligo(dT)30-Latex and PCR: identification of cellular genes which are overexpressed in senescent human diploid fibroblasts.Anal Biochem. 1993 Oct;214(1):58-64. doi: 10.1006/abio.1993.1456. Anal Biochem. 1993. PMID: 8250255
-
Subtractive cDNA cloning using oligo(dT)30-latex and PCR: isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells.Nucleic Acids Res. 1991 Dec;19(25):7097-104. doi: 10.1093/nar/19.25.7097. Nucleic Acids Res. 1991. PMID: 1766870 Free PMC article.
-
A modified cDNA subtraction to identify differentially expressed genes from plants with universal application to other eukaryotes.Anal Biochem. 2005 Oct 1;345(1):149-57. doi: 10.1016/j.ab.2005.07.029. Anal Biochem. 2005. PMID: 16137632
-
[cDNA subtraction].Tanpakushitsu Kakusan Koso. 1993 Feb;38(3):482-8. Tanpakushitsu Kakusan Koso. 1993. PMID: 8488282 Review. Japanese. No abstract available.
-
Principles of DNA cloning.BMJ. 1989 Oct 21;299(6706):1019-22. doi: 10.1136/bmj.299.6706.1019. BMJ. 1989. PMID: 2556195 Free PMC article. Review. No abstract available.