Constraction of a subtracted cDNA library using oligo(dT)-latex
- PMID: 2101904
Constraction of a subtracted cDNA library using oligo(dT)-latex
Abstract
An efficient method for constraction of subtracted cDNA library was developed using oligo(dT 30. Latex and PCR. This method improved the chances for identifying cDNA clones corresponding to scarce class of mRNA that is expressed differentially during cellular growth and differentiation.