Isolation of ancestral sylvatic dengue virus type 1, Malaysia

Abstract

Ancestral sylvatic dengue virus type 1, which was isolated from a monkey in 1972, was isolated from a patient with dengue fever in Malaysia. The virus is neutralized by serum of patients with endemic DENV-1 infection. Rare isolation of this virus suggests a limited spillover infection from an otherwise restricted sylvatic cycle.

Figure

Maximum clade credibility tree of complete envelope genes of dengue virus type 1 (DENV-1) isolates. Horizontal branches are drawn to a scale of estimated year of divergence. Coalescent times with 95% highest posterior density values (ranges in parentheses) and posterior probability values (all 1.0) of key nodes are shown. Patient convalescent-phase serum samples used for neutralization assays from which virus was isolated are indicated at the end of branches according to their virus groups. Box indicates sylvatic DENV-1 isolated in the study. New sequences were used to create the phylogenetic tree are as in Table 1. SII, subgenotype II, SI, subgenotype I.