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. 2010 Oct 27;15(11):7547-57.
doi: 10.3390/molecules15117547.

Free radical scavenging activity and characterization of sesquiterpenoids in four species of Curcuma using a TLC bioautography assay and GC-MS analysis

Jing Zhao  1 , Jiang-Sheng Zhang, Bin Yang, Guang-Ping Lv, Shao-Ping Li
Affiliations

Free radical scavenging activity and characterization of sesquiterpenoids in four species of Curcuma using a TLC bioautography assay and GC-MS analysis

Jing Zhao et al. Molecules. .

Abstract

The sesquiterpenoids are one of major groups of antioxidants in Curcuma besides curcuminoids. However, the real substances contributing to the antioxidant activity are still unknown. In this paper, the antioxidant activity of sesquiterpenoids in four species and two essential oils from Curcuma genus was determined and compared based on TLC separation and DPPH bioautography assay. Their antioxidant capacities were quantitatively evaluated using densitometry with detection at 530 nm (λ(reference )= 800 nm) using vitamin C as reference. The results showed that Curcuma longa rhizomes had the highest antioxidant capacity while C. phaeocaulis presented the lowest one among the four species of Curcuma. Moreover, essential oil of C. wenyujin showed higher antioxidant potential than that of C. longa. The main TLC bands with antioxidant activity of the four species of Curcuma were collected and characterized using GC-MS, and thus curzerene, furanodiene, α-turmerone, β-turmerone and β-sesquiphellandrene were determined as major sesquiterpenoids with antioxidant activity in Curcuma.

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Figures

Figure 1
Figure 1
Typical TLC photography of methanol extracts of rhizomes from four species of Curcuma (A), essential oils of C. wenyujin (B, EOCW) and C. longa (C, EOCL) colorized with 0.04%DPPH.
Figure 2
Figure 2
Typical TLC densitograms of vitamin C (A), mixed standards (B), methanol extracts of C. wenyujin (C), C. kwangsiensis (D), C. phaeocaulis (E) and C. longa (F) colorized with 0.04%DPPH.
Figure 3
Figure 3
The sliced raw materials of C. kwangsiensi (A), C. phaeocaulis (B), C. wenyujin (C) and C. longa (D).
See this image and copyright information in PMC

References

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