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. 1990 Jul;15(1):169-71.
doi: 10.1007/BF00017737.

Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction

R D'Ovidio  1 , O A Tanzarella, E Porceddu
Affiliations

Rapid and efficient detection of genetic polymorphism in wheat through amplification by polymerase chain reaction

R D'Ovidio et al. Plant Mol Biol. 1990 Jul.

Abstract

The polymerase chain reaction (PCR) was used to amplify genomic DNA of several wheat genotypes. The oligonucleotides used as primers were the terminal sequences of a gamma-gliadin gene. The electrophoretic analysis of the PCR products showed specific bands which revealed both inter- and intra-specific genetic polymorphism among the examined genotypes. The technique is proposed as a very simple and efficient alternative to RFLP markers.

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References

    1. Hum Genet. 1989 Aug;83(1):1-15 - PubMed

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