Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Dec;43(6):579-83.
doi: 10.1111/j.1365-2184.2010.00707.x.

Chromatin-bound PCNA as S-phase marker in mononuclear blood cells of patients with acute lymphoblastic leukaemia or multiple myeloma

F Zölzer  1 , O Basu, P U Devi, S P Mohanty, C Streffer
Affiliations

Chromatin-bound PCNA as S-phase marker in mononuclear blood cells of patients with acute lymphoblastic leukaemia or multiple myeloma

F Zölzer et al. Cell Prolif. 2010 Dec.

Abstract

Objectives: Proliferating cell nuclear antigen (PCNA) has often been used as a marker to aid assessment of tumour growth fraction. This paper addresses the question of whether it can be used as an S-phase marker, when the non-chromatin-bound form of the protein is removed by pepsin treatment.

Materials and methods: Cytofluorometric measurements were carried out after immunofluorescence staining of PCNA and counterstaining of DNA. S-phase fraction was determined with the help of windows on PCNA versus DNA scattergrams, or mathematically from DNA histograms.

Results: S-phase fractions obtained using the two methods correlated well, but did not always agree, exact discrepancies depending on the mathematical model used for histogram analysis.

Conclusions: Determination of S-phase fractions with the help of PCNA immunofluorescence staining is possible, and probably more reliable than calculation of S-fractions from DNA histograms. It thus offers an alternative to assays involving BrdU labelling in vivo.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Representative scattergrams of PCNA‐labelled cells from ALL patients (upper panel: aneuploid tumour, lower panel: diploid tumour).
Figure 2
Figure 2
Correlation between PCNA‐positive fractions and S‐phase fractions from DNA histograms for the three subsets of patients (ALL – peripheral blood, ALL – bone marrow, MM – bone marrow).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Bravo R, Frank R, Blundell PA, Macdonald‐Bravo H (1987) Cyclin/PCNA is the auxiliary protein of DNA‐polymerase‐δ. Nature 326, 515–517. - PubMed
    1. Prelich G, Tan CK, Kostura M, Mathews MB, So AG, Downey KM et al. (1987) Functional identity of proliferating cell nuclear antigen and DNA polymerase δ auxiliary protein. Nature 326, 517–520. - PubMed
    1. Nakayasu H, Berezney R (1989) Mapping replicational sites in the eucaryotic cell nucleus. J. Cell Biol. 108, 1–11. - PMC - PubMed
    1. Somanathan S, Suchya TM, Siegel AJ, Berezney R (2001) Targeting of PCNA replication in the mammalian cell nucleus. J. Cell. Biochem. 81, 56–67. - PubMed
    1. Masuda Y, Suzuki M, Piao J, Gu Y, Tsurimoto T, Kamiya K (2007) Dynamics of human replication factors in the elongation phase of DNA replication. Nucleic Acids Res. 35, 6904–6916. - PMC - PubMed

MeSH terms