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. 2010 Nov 1;2010(11):pdb.prot5516.
doi: 10.1101/pdb.prot5516.

DNA extraction from freeze-dried plant tissue with CTAB in a 96-well format

An-Ping Hsia, Hsin D Chen, Kazuhiro Ohtsu, Patrick S Schnable

DNA extraction from freeze-dried plant tissue with CTAB in a 96-well format

An-Ping Hsia et al. Cold Spring Harb Protoc. .

Abstract

This protocol is a modified version of DNA isolation using cetyltrimethylammonium bromide (CTAB) and 96-well plates. It is high-throughput, which facilitates the analysis of large mapping populations. The DNA yield is adequate for at least 100-500 polymerase chain reaction (PCR) procedures.

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