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. 2011 Jan;39(Database issue):D342-6.
doi: 10.1093/nar/gkq1072. Epub 2010 Nov 2.

ThYme: a database for thioester-active enzymes

David C Cantu  1 , Yingfei Chen, Matthew L Lemons, Peter J Reilly
Affiliations

ThYme: a database for thioester-active enzymes

David C Cantu et al. Nucleic Acids Res. 2011 Jan.

Abstract

The ThYme (Thioester-active enzYme; http://www.enzyme.cbirc.iastate.edu) database has been constructed to bring together amino acid sequences and 3D (tertiary) structures of all the enzymes constituting the fatty acid synthesis and polyketide synthesis cycles. These enzymes are active on thioester-containing substrates, specifically those that are parts of the acyl-CoA synthase, acyl-CoA carboxylase, acyl transferase, ketoacyl synthase, ketoacyl reductase, hydroxyacyl dehydratase, enoyl reductase and thioesterase enzyme groups. These groups have been classified into families, members of which are similar in sequences, tertiary structures and catalytic mechanisms, implying common protein ancestry. ThYme is continually updated as sequences and tertiary structures become available.

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Figures

Figure 1.
Figure 1.
The fatty acid synthesis cycle and the enzyme groups that are part of it. ACC: acetyl-CoA carboxylase; ACS: acyl-CoA synthase; AT: acyl transferase; ER: enoyl reductase; HD: hydroxyacyl dehydratase; KR: ketoacyl reductase; KS: ketoacyl synthase; MKS: methylketone synthase; TE: thioesterase. SX: Coenzyme A or acyl carrier protein.
Figure 2.
Figure 2.
A typical family homepage in ThYme.
See this image and copyright information in PMC

References

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