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Comparative Study
. 2011 Jan;50(1):42-5.
doi: 10.1016/j.jcv.2010.09.022. Epub 2010 Nov 2.

Evaluation of commercial ResPlex II v2.0, MultiCode-PLx, and xTAG respiratory viral panels for the diagnosis of respiratory viral infections in adults

Joan-Miquel Balada-Llasat  1 , Heidi LaRue, Cheryl Kelly, Lisa Rigali, Preeti Pancholi
Affiliations
Comparative Study

Evaluation of commercial ResPlex II v2.0, MultiCode-PLx, and xTAG respiratory viral panels for the diagnosis of respiratory viral infections in adults

Joan-Miquel Balada-Llasat et al. J Clin Virol. 2011 Jan.

Abstract

Background: Commercial multiplex PCR panels for respiratory viruses (PRV) have been recently developed. ResPlex II Panel v2.0 (Qiagen), MultiCode-PLx (EraGen Biosciences), and xTAG (Luminex) PRV's were studied. All assays detect influenza A and B, adenovirus, parainfluenza 1-3, respiratory syncytial virus A and B, human metapneumovirus and human rhinovirus. The ResPlex II additionally detects coronavirus (229E, OC43, NL63, HKU1), coxsackie/echo virus, bocavirus and differentiates adenoviruses (B, E). The MultiCode-PLX assay detects 229E, OC43, and NL63, differentiates parainfluenza 4a, 4b and adenoviruses (B, C, E). The xTAG additionally subtypes influenza A as seasonal H1 and H3.

Study design: 202 specimens collected from adult patients with signs of respiratory infection from November, 2008 to May, 2009 were used for evaluating the performance of the three commercial PRV assays. Viral culture and xTAG were used as the standards to assess sensitivity and specificity.

Results: The PRV assays detected more viruses than culture. When compared to culture, the xTAG PRV showed a sensitivity and specificity of 100% and 91%, compared to MultiCode-PLx with 89% and 87%, and ResPlex II with 89% and 94%, respectively. Co-infection was detected in a small subset of patient specimens. Each panel showed differences in sensitivities for individual viruses.

Conclusions: While the ResPlex II and MultiCode-PLx offer a broader virus detection range and greater ease of use, the xTAG PRV showed increased sensitivity to common viral targets represented in the assays, and also had the ability to differentiate human from non-human influenza A H1.

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References

    1. van Woensel J.B., van Aalderen W.M., Kimpen J.L. Viral lower respiratory tract infection in infants and young children. BMJ. 2003;327(7405):36–40. - PMC - PubMed
    1. Dong J., Olano J.P., McBride J.W., Walker D.H. Emerging pathogens: challenges and successes of molecular diagnostics. J Mol Diagn. 2008;10(3):185–197. - PMC - PubMed
    1. Ginocchio C.C., Zhang F., Manji R., Arora S., Bornfreund M., Falk L. Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak. J Clin Virol. 2009;45(3):191–195. - PMC - PubMed
    1. Chemaly R.F., Ghosh S., Bodey G.P., Rohatgi N., Safdar A., Keating M.J. Respiratory viral infections in adults with hematologic malignancies and human stem cell transplantation recipients: a retrospective study at a major cancer center. Medicine (Baltimore) 2006;85(5):278–287. - PubMed
    1. Garbino J., Gerbase M.W., Wunderli W., Deffernez C., Thomas Y., Rochat T. Lower respiratory viral illnesses: improved diagnosis by molecular methods and clinical impact. Am J Respir Crit Care Med. 2004;170(11):1197–1203. - PubMed