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Case Reports
. 2010 Dec;12(6):513-7.
doi: 10.1111/j.1399-3062.2010.00582.x. Epub 2010 Nov 10.

Early emergence of an H275Y mutation in a hematopoietic cell transplant recipient treated with intravenous peramivir

C Renaud  1 , S A Pergam, C Polyak, R Jain, J Kuypers, J A Englund, L Corey, M J Boeckh
Affiliations
Case Reports

Early emergence of an H275Y mutation in a hematopoietic cell transplant recipient treated with intravenous peramivir

C Renaud et al. Transpl Infect Dis. 2010 Dec.

Abstract

Oseltamivir resistance in pandemic 2009 influenza A/H1N1 is caused by the neuraminidase mutation H275Y. This mutation has also been associated with in vitro resistance to peramivir, but few clinical cases have been described to date. Using allele-specific real-time reverse transcriptase polymerase chain reaction assay for the H275Y mutation, we were able to identify resistant H1N1 in a hematopoietic cell transplant recipient receiving intravenous peramivir therapy, and through serial testing we determined the molecular evolution of resistance. This case demonstrates that an H275Y mutant population can emerge early and replicate in vivo under peramivir antiviral pressure to become the major viral population.

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Figures

Fig 1
Fig 1. Clinical course and percentage of pandemic H1N1 population with H275Y mutation
*log10 copies/reaction. BAL, bronchoalveolar lavage; NP, nasopharyngeal; asp, aspirate; IV, intravenous; Neg, negative.
See this image and copyright information in PMC

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