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. 2010 Jul 31;4(3):577-84.
doi: 10.1007/s12072-010-9197-z.

A case-control study on sequence variations in the enhancer II/core promoter/precore and X genes of hepatitis B virus in patients with hepatocellular carcinoma

Pisit Tangkijvanich, Pattaratida Sa-Nguanmoo, Varocha Mahachai, Apiradee Theamboonlers, Yong Poovorawan

A case-control study on sequence variations in the enhancer II/core promoter/precore and X genes of hepatitis B virus in patients with hepatocellular carcinoma

Pisit Tangkijvanich et al. Hepatol Int. .

Abstract

Purpose: To evaluate the sequence variations in the enhancer II (EnhII)/basal core promotor (BCP)/precore (PC) and X genes of hepatitis B virus (HBV) in Thai patients with hepatocellular carcinoma (HCC) by conducting a cross-sectional case-control study.

Methods: As much as 60 patients with HCC and 60 patients without HCC, who were matched for sex, age, hepatitis B e antigen (HBeAg) status, and HBV genotype, were included. Viral mutations in the EnhII/BCP/PC and X regions were characterized by direct sequencing in serum samples.

Results: The prevalence of T1753C/A, A1762T/G1764A and G1899A mutations were significantly higher in the HCC group compared to the non-HCC group (43.3 vs. 23.3%, P = 0.02; 88.3 vs. 53.0%, P < 0.001; and 35.0 vs. 8.3%, P = 0.001, respectively). No significant difference between groups was found with respect to G1613A, C1653T, C1766T/T1768A, A1846T/C, T1858C, and G1896A mutations. By multiple logistic regression analysis, the presence of cirrhosis, A1762T/G1764A and G1899A mutations were independently associated with the risk of HCC.

Conclusion: These data suggested that A1762T/G1764A and G1899A mutations were associated with the development of HCC in Thai patients.

Keywords: Core promoter; Enhancer II; Hepatitis B virus; Hepatocellular carcinoma; Precore; X genes.

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