Effect of heparin on bone formation in cultured fetal rat calvaria
- PMID: 2106377
- DOI: 10.1007/BF02555042
Effect of heparin on bone formation in cultured fetal rat calvaria
Abstract
To assess the effects of heparin on bone formation we measured [3H]proline incorporation into collagenase-digestible (CDP) and noncollagen protein (NCP), [3H]thymidine (TdR) incorporation into DNA, and DNA content in 21-day-old fetal rat calvaria cultured in BGJ medium with bovine serum albumin for 24-96 hours. Heparin at 5-125 micrograms/ml decreased TdR incorporation by 26-51% at 24 and 96 hours. At 96 hours, heparin 5, 25, and 125 micrograms/ml decreased [3H]proline incorporation into CDP by 41, 48, and 32%, respectively, with no significant change in NCP. To evaluate the possible role of PGE2 in these inhibitory responses, media PGE2 concentration was measured and the effects of heparin on CDP labeling and DNA synthesis were tested in the presence of indomethacin, piroxicam, and flurbiprofen to inhibit endogenous prostaglandin E2 (PGE2) production and in the presence of a high concentration (10(-7) M) of exogenous PGE2. Heparin did not alter PGE2 production at 24 hours but at 48 hours there was a significant reduction. At 96 hours, indomethacin (10(-6) M) inhibited [3H]-proline incorporation into CDP by 38% but had no effect on the labeling of NCP. Heparin had no further significant inhibitory effect in the presence of indomethacin. Piroxicam and flurbiprofen did not alter DNA content and had a smaller inhibitory effect than indomethacin on the labeling of CDP. Moreover, addition of heparin produced a further inhibition of CDP and DNA content and finally, heparin decreased CDP labeling by 71% in the presence of PGE2.(ABSTRACT TRUNCATED AT 250 WORDS)
Similar articles
-
Biphasic effects of prostaglandin E2 on bone formation in cultured fetal rat calvariae: interaction with cortisol.Endocrinology. 1990 Mar;126(3):1654-9. doi: 10.1210/endo-126-3-1654. Endocrinology. 1990. PMID: 2155107
-
Interactive effects of basic fibroblast growth factor and heparin on bone in 21-day fetal rat calvariae.Conn Med. 1993 Jan;57(1):9-11. Conn Med. 1993. PMID: 8449008
-
Effects of ethanol and acetaldehyde on collagen synthesis, prostaglandin release and resorption of fetal rat bone in organ culture.Bone. 1990;11(1):47-51. doi: 10.1016/8756-3282(90)90071-6. Bone. 1990. PMID: 2331431
-
Effects of transforming growth factor alpha and interleukin-1 on DNA synthesis, collagen synthesis, procollagen mRNA levels, and prostaglandin E2 production in cultured fetal rat calvaria.J Bone Miner Res. 1989 Oct;4(5):731-6. doi: 10.1002/jbmr.5650040512. J Bone Miner Res. 1989. PMID: 2816517
-
Simultaneous assessment of bone resorption and formation in cultures of 22-day fetal rat parietal bones: effects of parathyroid hormone and prostaglandin E2.Bone. 1990;11(1):61-5. doi: 10.1016/8756-3282(90)90073-8. Bone. 1990. PMID: 2331433
Cited by
-
Effects of short-term, high dose, heparin therapy on biochemical markers of bone metabolism.Clin Rheumatol. 1995 Nov;14(6):663-6. doi: 10.1007/BF02207933. Clin Rheumatol. 1995. PMID: 8608685
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Medical