Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1990 Mar;87(5):1947-9.
doi: 10.1073/pnas.87.5.1947.

Sequencing of peptides and proteins with blocked N-terminal amino acids: N-acetylserine or N-acetylthreonine

Affiliations
Comparative Study

Sequencing of peptides and proteins with blocked N-terminal amino acids: N-acetylserine or N-acetylthreonine

D Wellner et al. Proc Natl Acad Sci U S A. 1990 Mar.

Erratum in

  • Proc Natl Acad Sci U S A 1990 Jul;87(14):5587

Abstract

Many proteins cannot be directly sequenced by Edman degradation because they have a blocked N-terminal residue. A method is presented for deblocking such proteins when the N-terminal residue is N-acetylserine (which occurs frequently in eukaryotic proteins) or N-acetylthreonine. The method has been applied successfully to the determination of the N-terminal amino acid sequence of human, bovine, and rat parathymosins. Prothymosin alpha and other blocked proteins and peptides were also readily deblocked and sequenced by this procedure. It is proposed that the mechanism of the deblocking reaction involves an acid-catalyzed N----O shift of the acetyl group followed by a beta-elimination.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Proc Natl Acad Sci U S A. 1985 Jan;82(1):73-7 - PubMed
    1. Biochem J. 1966 May;99(2):356-66 - PubMed
    1. Biochemistry. 1968 Feb;7(2):516-21 - PubMed
    1. Biochem Biophys Res Commun. 1974 May 7;58(1):250-6 - PubMed
    1. J Biol Chem. 1976 Feb 25;251(4):1009-14 - PubMed

Publication types

LinkOut - more resources