Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Dec;41 Suppl 2(Suppl 2):150-3.
doi: 10.1111/j.1365-2052.2010.02125.x.

Identification of equine major histocompatibility complex haplotypes using polymorphic microsatellites

C T Tseng  1 , D Miller, J Cassano, E Bailey, D F Antczak
Affiliations

Identification of equine major histocompatibility complex haplotypes using polymorphic microsatellites

C T Tseng et al. Anim Genet. 2010 Dec.

Abstract

A system for identifying equine major histocompatibility complex (MHC) haplotypes was developed based on five polymorphic microsatellites located within the MHC region on ECA 20. Molecular signatures for 50 microsatellite haplotypes were recognized from typing 353 horses. Of these, 23 microsatellite haplotypes were associated with 12 established equine leucocyte antigen (ELA) haplotypes in Thoroughbreds and Standardbreds. Five ELA serotypes were associated with multiple microsatellite subhaplotypes, expanding the estimates of diversity in the equine MHC. The strong correlations between serological and microsatellite typing demonstrated a linkage to known MHC class I protein polymorphisms and validated this assay as a useful supplement to ELA serotyping, and in some applications, a feasible alternative method for MHC genotyping in horse families and in population studies.

PubMed Disclaimer

References

    1. Antczak DF, Bailey E, Barger B, Guerin G, Lazary S, McClure J, Mottironi VD, Symons R, Templeton J, Varewyck H. Joint report of the Third International Workshop on Lymphocyte Alloantigens of the Horse, Kennett Square, Pennsylvania, 25–27 April 1984. Animal Genetics. 1986;17:363–73. - PubMed
    1. Gustafson AL, Tallmadge RL, Ramlachan N, Miller D, Bird H, Antczak DF, Raudsepp T, Chowdhary BP, Skow LC. An ordered BAC contig map of the equine major histocompatibility complex. Cytogenetic and genome research. 2003;102:189–95. - PubMed
    1. Hughes AL, Nei M. Models of host-parasite interaction and MHC polymorphism. Genetics. 1992;132:863–4. - PMC - PubMed
    1. Lazary S, Antczak DF, Bailey E, Bell TK, Bernoco D, Byrns G, McClure JJ. Joint Report of the Fifth International Workshop on Lymphocyte Alloantigens of the Horse, Baton Rouge, Louisiana, 31 October-1 November 1987. Animal Genetics. 1988;19:447–56. - PubMed
    1. Tallmadge RL, Campbell JA, Miller DC, Antczak DF. Analysis of MHC class I genes across horse MHC haplotypes. Immunogenetics. 2010;62:159–72. - PMC - PubMed

Publication types