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Comparative Study
. 1990 Mar;9(3):741-7.
doi: 10.1002/j.1460-2075.1990.tb08168.x.

Beta-lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes

D J Smith  1 , M K Burnham, J H Bull, J E Hodgson, J M Ward, P Browne, J Brown, B Barton, A J Earl, G Turner
Affiliations
Comparative Study

Beta-lactam antibiotic biosynthetic genes have been conserved in clusters in prokaryotes and eukaryotes

D J Smith et al. EMBO J. 1990 Mar.

Abstract

A cosmid clone containing closely linked beta-lactam antibiotic biosynthetic genes was isolated from a gene library of Flavobacterium sp. SC 12,154. The location within the cluster of the DNA thought to contain the gene for delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS), the first step in the beta-lactam antibiotic biosynthetic pathway, was identified by a novel method. This DNA facilitated the isolation, by cross-hybridization, of the corresponding DNA from Streptomyces clavuligerus ATCC 27064, Penicillium chrysogenum Oli13 and Aspergillus nidulans R153. Evidence was obtained which confirmed that the cross-hybridizing sequences contained the ACVS gene. In each case the ACVS gene was found to be closely linked to other beta-lactam biosynthetic genes and constituted part of a gene cluster.

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References

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