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Review
. 2011 Jan;13(1):18-23.
doi: 10.1038/aja.2010.71. Epub 2010 Nov 15.

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Xiao-Yan Huang  1 , Jia-Hao Sha
Affiliations
Review

Proteomics of spermatogenesis: from protein lists to understanding the regulation of male fertility and infertility

Xiao-Yan Huang et al. Asian J Androl. 2011 Jan.

Abstract

Proteomic technologies have undergone significant development in recent years, which has led to extensive advances in protein research. Currently, proteomic approaches have been applied to many scientific areas, including basic research, various disease and malignant tumour diagnostics, biomarker discovery and other therapeutic applications. In addition, proteomics-driven research articles examining reproductive biology and medicine are becoming increasingly common. The key challenge for this field is to move from lists of identified proteins to obtaining biological information regarding protein function. The present article reviews the available scientific literature related to spermatogenesis. In addition, this study uses two-dimensional electrophoresis mass spectrometry (2DE-MS) and liquid chromatography (LC)-MS to construct a series of proteome profiles describing spermatogenesis. This large-scale identification of proteins provides a rich resource for elucidating the mechanisms underlying male fertility and infertility.

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Figures

Figure 1
Figure 1
Research strategies in our laboratory for designing spermatogenesis-related proteomics.
Figure 2
Figure 2
Partial graphs of two-dimensional (2DE) results from mouse adult testis. The red arrow indicates the protein spots with detailed information in the box.
Figure 3
Figure 3
Expression tendency of four proteins in our proteome profile respectively. APOA1 was highly expressed in spermatogonia, whereas it was downregulated during spermatogenesis. GSTM2 was highly expressed in primary spermatocytes, whereas the expression level was reduced with spermiogenesis. PGK2 and PRDX4 were upregulated during mouse spermatogenesis, and the highest expression level was found in spermatozoa. The vertical axis indicates expression levels of these proteins in our studies. The horizontal axis indicates different time points of mouse development stages. d, day.
See this image and copyright information in PMC

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