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. 2010 Dec 10;49(50):9635-9.
doi: 10.1002/anie.201005657.

Computed tomography in color: NanoK-enhanced spectral CT molecular imaging

Dipanjan Pan  1 , Ewald Roessl, Jens-Peter Schlomka, Shelton D Caruthers, Angana Senpan, Mike J Scott, John S Allen, Huiying Zhang, Grace Hu, Patrick J Gaffney, Eric T Choi, Volker Rasche, Samuel A Wickline, Roland Proksa, Gregory M Lanza
Affiliations

Computed tomography in color: NanoK-enhanced spectral CT molecular imaging

Dipanjan Pan et al. Angew Chem Int Ed Engl. .
No abstract available

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Figures

Figure 1
Figure 1
Synthesis and physicochemical characterization of NanoK: a) Preparation of bismuth-enriched K-edge nanocolloid (NanoK (Bi)): 1) Suspension of bismuth n-decanoate (1) in sorbitan sesquioleate, vigorously vortex and mixing, filter using cotton bed, vortex; 2) preparation of phospholipid thin film; 3) resuspension of the thin film in water (0.2 μM); 4) microfluidization at 4°C, 20000 psi, 4 min, dialysis (cellulosic membrane, MWCO 20 K). b) Characterization table for three replicates of NanoK. c) Hydrodynamic particle size distribution from DLS. d) Anhydrous state TEM images (staining: uranyl acetate; scale bar: 100 nm. e) AFM image (deposited on glass substrate).
Figure 2
Figure 2
Virtual conventional CT (a) and spectral CT (b) cross-sectional slices of serially diluted NanoK (green) and calcium (blue) phantoms. c) Variation of spectral CT signal (HU) with bismuth concentration; slope y =5.3353x−67.44; R2 =0.999. d) Cross-sectional slices of fibrin clot targeted with NanoK presenting calcium (scale: 0.1 mm). e) Pictorial representation of the vessel phantom. f) Gradient rendered image of fibrin clot targeted with NanoK (scale: 10 mm).
Figure 3
Figure 3
a,b) Lowest energy bin images with bismuth overlay and c,d) bismuth basis spectral CT images of human carotid specimen incubated with control nanocolloids and NanoK respectively. Note the complete absence of Bi in the control sample (a) and (c). Scale: diameter of the tube: 16 mm. Fibrin-targeted NanoK signal at the bottom of the tube represents thrombus dislodged from CEA during processing.
Figure 4
Figure 4
a) CT blood pool signal in rabbits following intravenous injection of NanoK. CT scan imaging parameters were thickness 0.8, increment 0.8, kV 90, mAs 1500, resolution HIGH, collimation 4 × 0.75, pitch 0.35, rotation time 1.5 s, FOV 75 mm. Inset: concentration of bismuth (ICP) in blood versus time post injection. Note that the background signal is at baseline in less than 30 min. b,c) Targeting in situ clot (thrombus) in rabbits; scale: maximum clot diameter =1.41 mm, minimum diameter =1.25 mm; see Figures S6 and S7. d) Two weeks clearance profile of bismuth from mice.
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