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. 2010 Jul;14(3):77-82.

Expression of ROR1 in patients with renal cancer--a potential diagnostic marker

Hodjattallah Rabbani  1 , Mahyar Ostadkarampour  1 , Amir Hossein Danesh Manesh  2 , Abbas Basiri  3 , Mahmood Jeddi-Tehrani  4 , Flora Forouzesh  5
Affiliations

Expression of ROR1 in patients with renal cancer--a potential diagnostic marker

Hodjattallah Rabbani et al. Iran Biomed J. 2010 Jul.

Abstract

Background: The ectopic expression of receptor tyrosine kinase Ror1 has been reported in patients with hematological malignancies such as chronic lymphocytic leukemia and acute lymphoblastic leukemia. Here we report, for the first time, expression of ROR1 gene in both tumor tissues and peripheral blood mononuclear cells (PBMC) from patients with renal cancer (RC).

Methods: In the current study, the expression of ROR1 gene was semi-quantitatively measured in PBMC and tumor tissues from 16 RC patients as well as PBMC from 22 healthy individuals relative to the expression of the housekeeping gene phosphoglucomutase 1 by RT-PCR.

Results: Our results showed that ROR1 was expressed at gene level in 81.3 percent of renal tumor tissues (13 out of 16) whereas it was expressed in 94 percent of PBMC from RC patients (15 out of 16). A weak expression of ROR1 was observed in PBMC of 4 out of 22 healthy individual. A significant expression of ROR1 was observed in PBMC from RC patients when compared to that in PBMC from normal healthy individuals (P less than 0.001). The expression of ROR1 in PBMC may reflect a shedding of tumor cells into blood stream.

Conclusion: We conclude that detection of a high level of ROR1 expression in blood cells might assist in early detection of renal malignancies, providing taking into consideration the clinical symptoms of the disease.

Keywords: ROR1; Ectopic expression; Renal cancer.

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Figures

Fig. 1
Fig. 1
RT-PCR of ROR1 and PGM1 genes. ROR1 and PGM1 PCR products related to each sample were electrophoresed together in equal volumes in the same wells of 1.5% ethidium bromide. (Upper panel) lanes 1 to 16: tissues of patient with RC. (Lower panel) lanes 1 to 16: PBMC from renal cancer patients. M, molecular weight marker VIII Roche (Roche Diagnostic, Mannheim, Germany).
Fig. 2
Fig. 2
Relative expression of ROR1 in 16 RC tissue samples. Dotted line shows the cut off level (4.2). The numbering corresponds to patients numbering in Table 1
Fig. 3.
Fig. 3.
Relative expression of ROR1 in PBMC of 16 renal cancer (RC) patients. Dotted line shows the cut off level (4.2). The numbering corresponds to patients numbering in Table 1.
Fig. 4
Fig. 4
Comparison of relative expression of ROR1 in healthy PBMC (n = 22), renal cancer (RC) patients´ PBMC (n = 16), and renal cancer (RC) patients´ tissues (n = 16). Dotted line shows the cut off level (4.2). Significant values are bolded
See this image and copyright information in PMC

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