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. 2010 Nov 16:5:20.
doi: 10.1186/1750-9378-5-20.

Human papillomavirus-16 presence and physical status in lung carcinomas from Asia

Francisco Aguayo  1 , Muhammad Anwar, Chihaya Koriyama, Andres Castillo, Quanfu Sun, Jacob Morewaya, Yoshito Eizuru, Suminori Akiba
Affiliations

Human papillomavirus-16 presence and physical status in lung carcinomas from Asia

Francisco Aguayo et al. Infect Agent Cancer. .

Abstract

Background: Although human papillomavirus (HPV) genome has been detected in lung cancer, its prevalence is highly variable around the world. Higher frequencies have been reported in far-east Asian countries, when compared with European countries. The present study analysed the HPV-16 presence in 60 lung carcinomas from the Asian countries China, Pakistan and Papua New Guinea.

Results: HPV-16 was present in 8/59 (13%) samples. According to histological type, HPV-16 was detected in 8/18 (44%) squamous cell carcinomas (SQCs), which were mainly from Pakistan; 0/38 (0%) adenocarcinomas (ACs), which were mainly from China; and in 0/4 (0%) small cell carcinomas (SCLCs). The observed histological difference was statistically significant (p < 0.001). HPV-16 viral load was also determined using real-time polymerase chain reaction (qRT-PCR); it ranged between 411 to 2345 copies/100 ng of genomic DNA. HPV-16 genome was found integrated into the host genome in every HPV-16 positive carcinoma.

Conclusion: These results support the notion that HPV-16 infection is highly associated with SQCs in Pakistan. Our results show a frequent HPV-16 integration in SQCs, although the low viral load casts doubt respect a direct etiological role of HPV in lung carcinomas from Asia. Additional HPV-16 characterization is necessary to establish a direct or indirect etiological role of HPV in this malignancy.

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Figures

Figure 1
Figure 1
HPV-16 is detected in lung carcinomas from Asia. DNA purified from lung carcinomas from Asian patients were amplified by PCR using A: generic primers flanking the HPV L1 region (65 bp) and B: specific primers flanking the HPV-16 E6 region (96 bp) and revealed by agarose gel electrophoresis and ethidium bromide staining. MW: 100 bp molecular weight marker; wells 1-7: positive clinical samples; wells 8-9: Negative and positive control (DNA from HPV-16 recombinant plasmid).
Figure 2
Figure 2
Quantitative Real-time PCR for HPV-16 in lung carcinomas from Asia. A: Amplification of HPV-16 E6 fragment; B: Melting analysis after E6 amplification; C: Amplification of HPV-16 E2 fragment; D: Melting analysis after E2 amplification.
See this image and copyright information in PMC

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