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Case Reports
. 1990 Apr;161(4):791-3.
doi: 10.1093/infdis/161.4.791.

Diagnosis of acute typhus infection using the polymerase chain reaction

M Carl  1 , C W Tibbs, M E Dobson, S Paparello, G A Dasch
Affiliations
Case Reports

Diagnosis of acute typhus infection using the polymerase chain reaction

M Carl et al. J Infect Dis. 1990 Apr.

Abstract

The first use of the polymerase chain reaction (PCR) for the diagnosis of an acute rickettsial infection is described. A primer pair derived from the 17-kDa antigen sequence of Rickettsia rickettsii gave specific amplification of a 434-base pair DNA fragment from the genome of Rocky Mountain spotted fever and endemic and epidemic typhus. The assay could detect as few as 30 rickettsiae. Detection of PCR-amplified DNA with a nonradioactive DNA probe confirmed an acute infection with Rickettsia prowazekii.

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