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. 2010 Nov 17:9:132.
doi: 10.1186/1476-511X-9-132.

A novel genetically-obese rat model with elevated 11 beta-hydroxysteroid dehydrogenase type 1 activity in subcutaneous adipose tissue

Sakamuri S S Vara Prasad  1 , Anamthathmakula Prashanth, Chodavarapu Pavan Kumar, Sirisha J Reddy, Nappan V Giridharan, Ayyalasomayajula Vajreswari
Affiliations

A novel genetically-obese rat model with elevated 11 beta-hydroxysteroid dehydrogenase type 1 activity in subcutaneous adipose tissue

Sakamuri S S Vara Prasad et al. Lipids Health Dis. .

Abstract

11 β-hydroxysteroid dehydrogenase type 1 (11 β-HSD1) catalyzes the conversion of inactive glucocorticoids to active glucocorticoids and plays an important role in the development of obesity and metabolic syndrome. 11 β-HSD1 activity is lower in liver and higher in omental adipose tissue of obese rodent models like obese zucker rats, Ob/Ob and db/db mice. Here, we report the 11 β-HSD1 activity in liver and adipose tissue of lean and obese rats of WNIN/Ob strain, a new genetic rat model of obesity. 11 β-HSD1 activity in liver, omental and subcutaneous adipose tissues of 3 month-old male WNIN/Ob lean and obese rats was assayed. As observed in other rodent models, 11 β-HSD1 activity was lower in liver and higher in omental adipose tissue. In contrast to other rodent obese models, WNIN/Ob obese rats had elevated 11 β-HSD1 activity in subcutaneous adipose tissue, which is in line with the observation in human obesity. Here, we conclude that dysregulation of 11 β-HSD1 in WNIN/Ob obese rat model is identical to human obesity, which makes it an excellent model for studying the effect of 11 β-HSD1 inhibitors in ameliorating obesity and metabolic syndrome.

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Figures

Figure 1
Figure 1
11β-HSD 1 activity in 3 month-old male WNIN/Ob lean and obese rats. (A). Omental fat. (B). Subcutaneous fat. (C). Liver. (D). Quadriceps muscle. Values are means ± S.E for 6 rats. Mean values with * mark are significant at P < 0.05 level (by student's t test). Comparisons were made between lean and obese phenotypes.
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References

    1. Rosmond R, Dallman MF, Bjorntorp P. Stress-related Cortisol secretion in men: relationships with abdominal obesity and endocrine, metabolic, and hemodynamic abnormalities. J Clin Endocrinol Metab. 1998;77:1853–1859. doi: 10.1210/jc.83.6.1853. - DOI - PubMed
    1. Tomilson JW, Walker EA, Bujalska IJ, Draper N, Lavery GG, Cooper MS, Hewison M, Stewart PM. 11beta-hydroxysteroid dehydrogenase type 1: A tissue-specific regulator of glucocorticoid response. Endo reviews. 2004;25:831–866. doi: 10.1210/er.2003-0031. - DOI - PubMed
    1. Draper N, Stewart PM. 11beta-hydroxysteroid dehydrogenase and the pre-receptor regulation of corticosteroid hormone action. J Endocrinol. 2005;186:251–271. doi: 10.1677/joe.1.06019. - DOI - PubMed
    1. Livingstone DEW, Jones GC, Smith K, Jamison PM, Andrew R, Kenyon CJ, Walker BR. Understanding the role of glucocorticoids in obesity: Tissue-specific alterations of corticosterone metabolism in obese zucker rats. Endocrinology. 2000;141:560–563. doi: 10.1210/en.141.2.560. - DOI - PubMed
    1. Rask E, Olsson T, Soderberg S, Andrew R, Livingstone DE, Johnson O, Walker BR. Tissue-specific dysregualtion of cortisol metabolism in human obesity. J Clin Endocrinol Metab. 2001;86:1418–1421. doi: 10.1210/jc.86.3.1418. - DOI - PubMed

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