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. 2010 Sep-Oct;18(5):503-8.
doi: 10.1590/s1678-77572010000500013.

Biocompatibility of orthodontic adhesives in rat subcutaneous tissue

Rogério Lacerda dos Santos  1 , Matheus Melo Pithon, Alline Birra Nolasco Fernandes, Márcia Grillo Cabral, Antônio Carlos de Oliveira Ruellas
Affiliations

Biocompatibility of orthodontic adhesives in rat subcutaneous tissue

Rogério Lacerda dos Santos et al. J Appl Oral Sci. 2010 Sep-Oct.

Abstract

Objective: The objective of the present study was to verify the hypothesis that no difference in biocompatibility exists between different orthodontic adhesives.

Material and methods: Thirty male Wistar rats were used in this study and divided into five groups (n=6): Group 1 (control, distilled water), Group 2 (Concise), Group 3 (Xeno III), Group 4 (Transbond XT), and Group 5 (Transbond plus Self-Etching Primer). Two cavities were performed in the subcutaneous dorsum of each animal to place a polyvinyl sponge soaked with 2 drops of the respective adhesive in each surgical loci. Two animals of each group were sacrificed after 7, 15, and 30 days, and their tissues were analyzed by using an optical microscope.

Results: At day 7, Groups 3 (Transbond XT) and 4 (Xeno III) showed intense mono- and polymorphonuclear inflammatory infiltrate with no differences between them, whereas Groups 1 (control) and 2 (Concise) showed moderate mononuclear inflammatory infiltrate. At day 15, severe inflammation was observed in Group 3 (Transbond XT) compared to other groups. At day 30, the same group showed a more expressive mononuclear inflammatory infiltrate compared to other groups.

Conclusion: Among the orthodontic adhesive analyzed, it may be concluded that Transbond XT exhibited the worst biocompatibility. However, one cannot interpret the specificity of the data generated in vivo animal models as a human response.

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Figures

Figure 2
Figure 2
Photomicrographs of histological samples after 7 days of implantation. A: evidence of acute inflammatory infiltrate with predominance of polymorphonuclear cells (1,000x magnification; scale: 100 μm). B: presence of granulation tissue (1,000x magnification; scale: 100 μm)
Figure 3
Figure 3
Photomicrographs of histological samples after 15 days of implantation. A: formation of granulomas with multinuclear giant cells (1,000x magnification; scale: 100 μm). B: areas of intense cell formation and deposition of collagen fibers (1,000x magnification; scale: 100 μm).
Figure 4
Figure 4
Photomicrographs of histological samples after 30 days of implantation: one can observe deposition of collagen fibers, thus indicating an ongoing repair process (1,000x magnification; scale: 100 μm)
See this image and copyright information in PMC

References

    1. al-Dawood A, Wennberg A. Biocompatibility of dentin bonding agents. Endod Dent Traumatol. 1993;9(1):1–7. - PubMed
    1. Axford SE, Ogden GR, Stewart AM, Saleh HA, Ross PE, Hopwood D. Fluid phase endocytosis within buccal mucosal cells of alcohol misusers. Oral Oncol. 1999;35(1):86–92. - PubMed
    1. Bouillaguet S, Wataha JC, Hanks CT, Ciucchi B, Holz J. In vitro cytotoxicity and dentin permeability of HEMA. J Endod. 1996;22(5):244–248. - PubMed
    1. Costa CA, Giro EM, Nascimento AB, Teixeira HM, Hebling J. Short-term evaluation of the pulpo-dentin complex response to a resin-modified glass-ionomer cement and a bonding agent applied in deep cavities. Dent Mater. 2003;19(8):739–746. - PubMed
    1. Costa CA, Hebling J, Hanks CT. Current status of pulp capping with dentin adhesive systems: a review. Dent Mater. 2000;16(3):188–197. - PubMed