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. 1990;116(2):134-40.
doi: 10.1007/BF01612667.

Intercellular variation in levels of adducts of aflatoxin B1 and G1 in DNA from rat tissues: a quantitative immunocytochemical study

C P Wild  1 , R Montesano, J Van Benthem, E Scherer, L Den Engelse
Affiliations

Intercellular variation in levels of adducts of aflatoxin B1 and G1 in DNA from rat tissues: a quantitative immunocytochemical study

C P Wild et al. J Cancer Res Clin Oncol. 1990.

Abstract

Adducts between aflatoxin B1 and G1 and DNA have been visualised and quantified in various rat tissues by a sensitive immunocytochemical approach. The quantitative validity of this assay has been examined by comparison with experiments using radioactively labelled aflatoxin. Rats were exposed to single and multiple doses of aflatoxin and a marked intercellular variation in adduct levels was observed in kidney and lung, in contrast to the liver, where binding was more homogeneous. No adducts were detected in the oesophagus, forestomach, colon, spleen or testes (detection limit approximately 300 pg aflatoxin/mg DNA). The DNA adduct data are discussed in relation to the carcinogenicity of aflatoxin B1 and G1.

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References

    1. Belinsky SA, White CM, Devereux TR, Swenberg JA, Anderson MW (1987) Cell selective alkylation of DNA in rat lung following low dose exposure to the tobacco specific carcinogen 4-(Nmethyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone. Cancer Res 47:1143–1148 - PubMed
    1. Busby WF, Wogan GN (1984) Aflatoxins. In: Searle CD (ed) Chemical carcinogens, ACS Monogr 182. Washington DC, pp 945–1136
    1. Butler WH, Greenblatt M, Lijinsky W (1969) Carcinogenesis in rats by aflatoxins B1, G1, and B2. Cancer Res 29:2206–2211 - PubMed
    1. Croy RG, Wogan GN (1981a) Quantitative comparison of covalent aflatoxin-DNA adducts formed in rat and mouse livers and kidneys. J Natl Cancer Inst 66:761–768 - PubMed
    1. Croy RG, Wogan GN (1981 b) Temporal patterns of covalent DNA adducts in rat liver after single and multiple doses of aflatoxin B1. Cancer Res 41:197–203 - PubMed

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