doi: 10.1021/ja107745g.
Epub 2010 Nov 19.
A selective NMR method for detecting choline containing compounds in liver tissue: the 1H-14N HSQC experiment
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- PMID: 21090567
- DOI: 10.1021/ja107745g
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A selective NMR method for detecting choline containing compounds in liver tissue: the 1H-14N HSQC experiment
J Am Chem Soc.
.
Abstract
The feasibility of a (1)H-(14)N HSQC experiment on tissues is demonstrated with a mouse liver based on the J couplings between the protons and the quadrupolar nucleus (14)N in choline. Free choline, phosphocholine, and glycerolphosphocholine (1)H-(14)N HSQC signals were selectively observed with all unwanted signals cleanly suppressed. The CH2O signals were well resolved in the two-dimensional spectrum, which can be used for quantitative analyses.
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