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. 2010 Dec 15;132(49):17349-51.
doi: 10.1021/ja107745g. Epub 2010 Nov 19.

A selective NMR method for detecting choline containing compounds in liver tissue: the 1H-14N HSQC experiment

Jiezhen Mao  1 , Ling Jiang, Bin Jiang, Maili Liu, Xi-an Mao
Affiliations

A selective NMR method for detecting choline containing compounds in liver tissue: the 1H-14N HSQC experiment

Jiezhen Mao et al. J Am Chem Soc. .

Abstract

The feasibility of a (1)H-(14)N HSQC experiment on tissues is demonstrated with a mouse liver based on the J couplings between the protons and the quadrupolar nucleus (14)N in choline. Free choline, phosphocholine, and glycerolphosphocholine (1)H-(14)N HSQC signals were selectively observed with all unwanted signals cleanly suppressed. The CH2O signals were well resolved in the two-dimensional spectrum, which can be used for quantitative analyses.

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