Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jan 15;55(1-2):135-8.
doi: 10.1016/0378-1097(90)90182-p.

Genetic transformation of intact cells of Bacillus subtilis by electroporation

P Brigidi  1 , E De Rossi, M L Bertarini, G Riccardi, D Matteuzzi
Affiliations

Genetic transformation of intact cells of Bacillus subtilis by electroporation

P Brigidi et al. FEMS Microbiol Lett. .

Abstract

Plasmid DNAs were introduced by electroporation into Bacillus subtilis PB1424 as an alternative to competent-cell or protoplast transformation. The maximum electroporation efficiency was 10(4) transformants/microgram DNA. Parameters including growth phase of cells, ionic strength of the suspending medium, concentration and size of plasmid DNAs, amplitude and duration of the pulse, were evaluated in order to determine conditions that improved transformation efficiency.

PubMed Disclaimer

LinkOut - more resources