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. 2011 Jan-Feb;3(1):17-20.
doi: 10.4161/mabs.3.1.14169. Epub 2011 Jan 1.

Sequencing antibody repertoires: the next generation

Nicolas Fischer  1
Affiliations

Sequencing antibody repertoires: the next generation

Nicolas Fischer. MAbs. 2011 Jan-Feb.

Abstract

Genomic studies have been revolutionized by the use of next generation sequencing (NGS) that delivers huge amounts of sequence information in a short span of time. The number of applications for NGS is rapidly expanding and significantly transforming many areas of life sciences. The field of antibody research and discovery is no exception. Several recent studies have harnessed the power of NGS for analyzing natural or synthetic immunoglobulin repertoires with unprecedented resolution and exploring alternative paths for antibody discovery. Thus, appreciating, and then exploiting, these advances is essential for staying at the edge of antibody innovation.

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Figures

Figure 1
Figure 1
Different applications of next generation sequencing to antibody research and discovery. Deep sequencing of natural immunoglobulin repertoires (A). Quality control of natural and synthetic antibody libraries (B). Alternative approaches for antibody discovery, bypassing screening and applicable to both in vitro selection and immunization strategies (B and C).
See this image and copyright information in PMC

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