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Comparative Study
. 2011 Feb;49(2):693-6.
doi: 10.1128/JCM.01463-10. Epub 2010 Nov 24.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains

A Bizzini  1 , K Jaton, D Romo, J Bille, G Prod'hom, G Greub
Affiliations
Comparative Study

Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains

A Bizzini et al. J Clin Microbiol. 2011 Feb.

Abstract

Conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identified with a valid score 45.9% of 410 clinical isolates from 207 different difficult-to-identify species having required 16S rRNA gene sequencing. MALDI-TOF MS might represent an alternative to 16S rRNA gene sequencing.

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Figures

Fig. 1.
Fig. 1.
Identification (ID) yield of MALDI-TOF MS analysis for 410 bacterial isolates identified at the species level by 16S rRNA gene sequencing.
See this image and copyright information in PMC

References

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