Serum antibody titers against heat shock protein 27 are associated with the severity of coronary artery disease

Abstract

Antibody titers to several heat shock proteins (anti-Hsps) have been reported to be associated with the severity and progression of cardiovascular disease. However, there are little data regarding anti-Hsp27 titers in patients with coronary artery disease (CAD). A total of 400 patients with suspected CAD were recruited. Based on the results of coronary angiography, these patients were classified into CAD(+) (n = 300) and CAD(-) (n = 100) groups defined as patients with ≥50% and <50% stenosis of any major coronary artery, respectively. Eighty-three healthy subjects were also recruited as the control group. Serum anti-Hsp27 IgG titers were measured using an in-house enzyme-linked immunosorbent assay. CAD(+) patients had significantly higher anti-Hsp27 titers compared with both CAD(-) and control groups. Anti-Hsp27 titers were also higher in the CAD(-) group compared with the control group. With regard to the number of affected vessels in the CAD(+) group, patients with three-vessel disease had higher anti-Hsp27 titers compared with both two-vessel disease (2VD) and one-vessel disease (1VD) subgroups. However, there was no significant difference between 1VD and 2VD subgroups. In multiple linear regression analysis, the number of narrowed vessels and smoking were significant independent determinants of serum anti-Hsp27 titers. The present findings indicate that serum anti-Hsp27 titers may be associated with the presence and severity of coronary artery disease.

Fig. 1

Serum anti-Hsp27 status in CAD⁺, CAD^-, and control groups. Compared with the control group: ***p < 0.001; compared with the CAD⁻ group: †††p < 0.001. Group comparisons were assessed using Kruskal–Wallis test (post hoc analyses were performed by paired samples t test)

Fig. 2

Serum anti-Hsp27 status in CAD⁺ subjects with 1-, 2- and 3VD. Compared with the 1VD group: *p < 0.05; compared to the 2VD group: †p < 0.05. Group comparisons were assessed using Kruskal–Wallis test (post hoc analyses were performed by paired samples t test)