Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Mar 2;133(8):2338-41.
doi: 10.1021/ja109044r. Epub 2010 Nov 29.

Production of lantipeptides in Escherichia coli

Yanxiang Shi  1 , Xiao Yang, Neha Garg, Wilfred A van der Donk
Affiliations
Free PMC article

Production of lantipeptides in Escherichia coli

Yanxiang Shi et al. J Am Chem Soc. .
Free PMC article

Abstract

Lantipeptides are ribosomally synthesized and posttranslationally modified peptides containing thioether cross-links. We describe the preparation of seven different lantipeptides in Escherichia coli and demonstrate that this methodology can be used to incorporate nonproteinogenic amino acids.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Posttranslational modifications in lantipeptide biosynthesis. Dha, dehydrolalanine; Dhb, dehydrobutyrine; Abu, 2-aminobutyric acid.
Figure 2
Figure 2
(A) Sequences of the ProcA lantipeptide core peptides used in this study. The residue replaced by pBpa in this work is highlighted in red. For the sequences of the leader peptides, see Figure S1. (B) Representative structures of prochlorosins 1.7 and 3.3. Abu, 2-aminobutyric acid; Dha, dehydroalanine; Dhb, dehydrobutyrine.
Figure 3
Figure 3
MALDI-MS spectra of precursor peptides modified by ProcM in E. coli and treated with a protease to remove the leader peptide. (A) ProcA1.7, (B) ProcA2.11, (C) ProcA3.3, and (D) ProcA3.2 Phe26pBpa.
Figure 4
Figure 4
(A) Sequences of the HalA core peptides. For the sequences of the leader peptides, see Figure S1. (B) Structures of haloduracin α and β. Abu, 2-aminobutyric acid; Dha, dehydroalanine; Dhb, dehydrobutyrine.
Figure 5
Figure 5
MALDI-MS spectra of precursor peptides modified in E. coli and treated with a protease to remove the leader peptide. (A) HalA1, (B) HalA2, and (C) NisA.
Figure 6
Figure 6
Antimicrobial assays against L. lactis HP. (1) authentic nisin; (2) nisin produced in E. coli; (3) Halα produced in E. coli combined with authentic Halβ; (4) authentic Halα combined with Halβ produced in E. coli; (5) authentic Halα and Halβ; (6) Halα and Halβ both produced in E. coli.
See this image and copyright information in PMC

References

    1. Goto Y.; Li B.; Claesen J.; Shi Y.; Bibb M. J.; van der Donk W. A. PLoS Biol. 2010, 8, e1000339. - PMC - PubMed
    1. Schnell N.; Entian K.-D.; Schneider U.; Götz F.; Zahner H.; Kellner R.; Jung G. Nature 1988, 333, 276–278. - PubMed
    1. Willey J. M.; van der Donk W. A. Annu. Rev. Microbiol. 2007, 61, 477–501. - PubMed
    1. Cortés J.; Appleyard A. N.; Dawson M. J. Methods Enzymol. 2009, 458, 559–574. - PubMed
    1. Levengood M. R.; Knerr P. J.; Oman T. J.; van der Donk W. A. J. Am. Chem. Soc. 2009, 131, 12024–12025. - PMC - PubMed

Publication types

MeSH terms