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. 1990 Jun;172(6):3496-9.
doi: 10.1128/jb.172.6.3496-3499.1990.

Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters

M A Farinha  1 , A M Kropinski
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Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters

M A Farinha et al. J Bacteriol. 1990 Jun.

Abstract

We have constructed a series of broad-host-range plasmids which use "visual screens" to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding beta-galactosidase and alkaline phosphatase from Escherichia coli and bacterial luciferase from Vibrio harveyi supply the promoterless indicator genes. The constructs were tested in E. coli and Pseudomonas aeruginosa.

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References

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