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. 1990 May;25(1):37-46.
doi: 10.1016/0165-2427(90)90108-5.

Production and characterization of monoclonal antibodies specific for bovine gamma-interferon

P R Wood  1 , J S Rothel, P G McWaters, S L Jones
Affiliations

Production and characterization of monoclonal antibodies specific for bovine gamma-interferon

P R Wood et al. Vet Immunol Immunopathol. 1990 May.

Abstract

Nine stable hybridoma cell lines were established which secreted specific monoclonal antibodies (MAbs) to bovine gamma-interferon (BoIFN-gamma). Specific binding of each of the MAbs to recombinant BoIFN-gamma (rBoIFN-gamma) was demonstrated in an indirect ELISA, whilst none of the MAbs bound to rBoIFN-alpha or rBoIFN-beta. In a Western blot the MAbs reacted with the 16 kDa and 32 kDa polypeptides present in rBoIFN-gamma preparations. Competitive ELISA's showed that four MAbs bound to one epitope on rBoIFN-gamma, and the other five MAbs bound to a separate epitope. Two MAbs, each recognising different epitopes, were shown to neutralise the anti-viral activity of natural BoIFN-gamma.

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