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. 2011 Feb;49(2):732-4.
doi: 10.1128/JCM.01970-10. Epub 2010 Dec 1.

Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

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Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

Marc Stegger et al. J Clin Microbiol. 2011 Feb.

Abstract

A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms.

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Figures

Fig. 1.
Fig. 1.
(A) Duplex PCR for CC398 sau1-hsdS1 and mecA detection. Lane M, 100-bp DNA ladder; lane 1, negative control (water); lane 2, non-CC398 methicillin-susceptible S. aureus (strain ATCC 6538); lane 3, non-CC398 methicillin-resistant S. aureus (strain ATCC 33591); lane 4, CC398 methicillin-sensitive S. aureus (SSI 52615); lane 5, CC398 methicillin-resistant S. aureus (KVL 288). (B) Real-time PCR melting curve analysis showing the melting temperature (Tm) of the amplicons. (−R′(T)), negative derivative of fluorescence with respect to temperature.

References

    1. Bens C. C., Voss A., Klaassen C. H. 2006. Presence of a novel DNA methylation enzyme in methicillin-resistant Staphylococcus aureus isolates associated with pig farming leads to uninterpretable results in standard pulsed-field gel electrophoresis analysis. J. Clin. Microbiol. 44:1875–1876 - PMC - PubMed
    1. Cockfield J. D., Pathak S., Edgeworth J. D., Lindsay J. A. 2007. Rapid determination of hospital-acquired meticillin-resistant Staphylococcus aureus lineages. J. Med. Microbiol. 56:614–619 - PubMed
    1. European Food Safety Authority 2009. Analysis of the baseline survey on the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in holdings with breeding pigs, in the EU, 2008, part A: MRSA prevalence estimates. Eur. Food Saf. Authority 7:1376 doi:10.2903/j.efsa.2009.1376. http://www.efsa.europa.eu/en/scdocs/doc/1376.pdf - DOI - PMC - PubMed
    1. Feil E. J., Li B. C., Aanensen D. M., Hanage W. P., Spratt B. G. 2004. eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J. Bacteriol. 186:1518–1530 - PMC - PubMed
    1. Harmsen D., et al. 2003. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J. Clin. Microbiol. 41:5442–5448 - PMC - PubMed

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