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. 1978 Aug;27(2):307-12.
doi: 10.1128/JVI.27.2.307-312.1978.

Presence of free viral DNA in simian virus 40-transformed nonproducer cells

L Daya-Grosjean, R Monier

Presence of free viral DNA in simian virus 40-transformed nonproducer cells

L Daya-Grosjean et al. J Virol. 1978 Aug.

Abstract

Extracts from several simian virus 40 (SV40)-transformed nonproducer cells were prepared by the hot-phenol procedure normally used to extract cellular RNA. These extracts contained SV40 infectious units. Part of the infectious units were identified as SV40 form I DNA molecules. The results of reconstruction experiments suggest that SV40 form I DNA is extractable by the hot-phenol procedure because of its fast renaturation rate. The significance of the presence of free viral DNA in nonproducer transformed cells is discussed.

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