Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Aug;27(2):436-42.
doi: 10.1128/JVI.27.2.436-442.1978.

Chromatographic separation of the polyoma virus proteins and renaturation of the isolated VP1 major capsid protein

J N Brady, R A Consigli

Chromatographic separation of the polyoma virus proteins and renaturation of the isolated VP1 major capsid protein

J N Brady et al. J Virol. 1978 Aug.

Abstract

Treatment of purified polyoma virions with 6 M guanidine-hydrochloride and 0.01 M beta-mercaptoethanol resulted in the immediate loss of both hemagglutinating and plaque-forming ability. Gel filtration through Sepharose CL-6B beads allowed separation of the dimer, VP1, VP2, VP3, and histone proteins VP4-7 in highly purified form. Renaturation of the purified VP1 protein resulted in the formation of subunits that were morphologically, biophysically, and immunologically similar to native virion capsomeres.

PubMed Disclaimer

References

    1. Biochem J. 1940 Sep;34(8-9):1248-57 - PubMed
    1. Proc Soc Exp Biol Med. 1960 Jan;103:250-2 - PubMed
    1. J Virol. 1978 Jul;27(1):193-204 - PubMed
    1. J Virol. 1977 Mar;21(3):1113-20 - PubMed
    1. J Virol. 1977 Sep;23(3):717-24 - PubMed

Publication types

MeSH terms

LinkOut - more resources