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. 2011 Feb;10(2):272-5.
doi: 10.1128/EC.00133-10. Epub 2010 Dec 3.

Interaction between the Candida albicans high-osmolarity glycerol (HOG) pathway and the response to human beta-defensins 2 and 3

Silvia Argimón  1 , Saranna Fanning, Jill R Blankenship, Aaron P Mitchell
Affiliations

Interaction between the Candida albicans high-osmolarity glycerol (HOG) pathway and the response to human beta-defensins 2 and 3

Silvia Argimón et al. Eukaryot Cell. 2011 Feb.

Abstract

Human β-defensins 2 and 3 are small cationic peptides with antimicrobial activity against the fungal pathogen Candida albicans. We found that hog1 and pbs2 mutants were hypersensitive to treatment with these peptides, pointing to a role of the high-osmolarity glycerol (HOG) pathway in the response to defensin-induced cell injury.

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Figures

Fig. 1.
Fig. 1.
(A) hBD-2 at the indicated concentrations inhibits the growth of reference strain DAY286, grown on rich medium, under our assay conditions as determined with a microplate reader. The y axis measures OD600 values. Error bars represent the standard deviations of results from three wells. (B to D) pbs2 (B), hog1 (C), and swe1 (D) mutant cells are hypersensitive to hBD-2. Error bars represent the ranges between results for at least two isolates of each mutant.
Fig. 2.
Fig. 2.
pbs2 and hog1 cells are hypersensitive to hBD-2 (A) and hBD-3 (B).
Fig. 3.
Fig. 3.
Hog1 and Pbs2 interaction is augmented in a VCI assay after treatment with hBD-2 and hBD-3.
See this image and copyright information in PMC

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