Review
doi: 10.1002/prca.200900138.
Epub 2009 Nov 19.
Combining laser capture microdissection and proteomics: methodologies and clinical applications
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- PMID: 21137037
- DOI: 10.1002/prca.200900138
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Review
Combining laser capture microdissection and proteomics: methodologies and clinical applications
Proteomics Clin Appl.
2010 Feb.
Abstract
Laser capture microdissection (LCM) has become an important tool for biomedical research. Various molecular biology techniques have been combined with LCM to reveal molecular profiles at an unprecedented tissue resolution. Proteomics is among those techniques that have proved fruitful in combination with LCM. This review provides an overview of the various proteomic techniques that have been developed for analyzing cells obtained using LCM. Methods for analyzing microdissected cells for various proteomic techniques are described and compared. In addition, various applications based on LCM and proteomics are discussed.
Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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