doi: 10.1093/nar/5.8.2861.
Purification of a DNA nicking-closing enzyme from mouse L cells
- PMID: 211488
- PMCID: PMC342212
- DOI: 10.1093/nar/5.8.2861
Item in Clipboard
Purification of a DNA nicking-closing enzyme from mouse L cells
Nucleic Acids Res.
1978 Aug.
Free PMC article
Abstract
A DNA nicking-closing enzyme has been purified from the nuclei of mouse L cells to 90% homogeneity. The denatured and reduced form of the enzyme has a molecular weight of 68,000 which is in agreement with the molecular weight of the native enzyme as determined by gel filtration and by sucrose sedimentation velocity assuming the protein is globular. Therefore, the active form of the enzyme is a monopolypeptide. Its isoelectric point is pH 4.2 +/- 0.2. The nicking-closing activity does not require a cofactor and does not involve any sulfhydryl group. The enzyme requires 0.2 M NaCl and pH in the range of 6.5-7.5 for optimal activity.
Similar articles
-
Eukaryotic DNA topoisomerases: two forms of type I DNA topoisomerases from HeLa cell nuclei.Proc Natl Acad Sci U S A. 1981 Jun;78(6):3487-91. doi: 10.1073/pnas.78.6.3487. Proc Natl Acad Sci U S A. 1981. PMID: 6267594 Free PMC article.
-
The purification and characterization of a DNA nicking-closing enzyme from Bacillus megaterium.Can J Biochem. 1978 Feb;56(2):123-8. doi: 10.1139/o78-020. Can J Biochem. 1978. PMID: 204401
-
Purification and characterization of wheat germ DNA topoisomerase I (nicking-closing enzyme).J Biol Chem. 1981 Jun 10;256(11):5860-5. J Biol Chem. 1981. PMID: 6263892
-
HeLa toposiomerase I.Methods Enzymol. 1983;100:133-7. Methods Enzymol. 1983. PMID: 6312254 No abstract available.
-
The predominant form of mammalian DNA topoisomerase I in vivo has a molecular mass of 100 kDa.Mol Biol Rep. 1994 Mar;19(2):99-103. doi: 10.1007/BF00997154. Mol Biol Rep. 1994. PMID: 8072496
Cited by
-
Drosophila topoisomerase I: isolation, purification and characterization.Nucleic Acids Res. 1982 Nov 11;10(21):6945-55. doi: 10.1093/nar/10.21.6945. Nucleic Acids Res. 1982. PMID: 6294622 Free PMC article.
-
DNA topoisomerase from Agrobacterium tumefaciens: purification and catalytic properties.Nucleic Acids Res. 1981 Feb 25;9(4):909-20. doi: 10.1093/nar/9.4.909. Nucleic Acids Res. 1981. PMID: 6262720 Free PMC article.
-
Elastic torsional strain in DNA within a fraction of SV40 minichromosomes: relation to transcriptionally active chromatin.EMBO J. 1982;1(11):1353-8. doi: 10.1002/j.1460-2075.1982.tb01322.x. EMBO J. 1982. PMID: 6327265 Free PMC article.
-
Eukaryotic DNA topoisomerases: two forms of type I DNA topoisomerases from HeLa cell nuclei.Proc Natl Acad Sci U S A. 1981 Jun;78(6):3487-91. doi: 10.1073/pnas.78.6.3487. Proc Natl Acad Sci U S A. 1981. PMID: 6267594 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
