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. 2010 Dec 21;16(47):5975-81.
doi: 10.3748/wjg.v16.i47.5975.

Relationship between COX-2 and cell cycle-regulatory proteins in patients with esophageal squamous cell carcinoma

Jun-Xing Huang  1 , Wei Xiao, Wei-Chang Chen, Mao-Song Lin, Zheng-Xiang Song, Ping Chen, Yun-Lei Zhang, Feng-Yue Li, Rong-Yu Qian, Eeva Salminen
Affiliations

Relationship between COX-2 and cell cycle-regulatory proteins in patients with esophageal squamous cell carcinoma

Jun-Xing Huang et al. World J Gastroenterol. .

Abstract

Aim: To investigate the correlation between cyclooxygenase-2 (COX-2) and cell cycle-regulatory proteins in patients with esophageal squamous cell carcinoma (ESCC).

Methods: One hundred and two surgically obtained specimens of ESCC were randomly collected. All specimens were obtained from patients who had not received chemo- or radiotherapy prior to surgical resection. Twenty-eight specimens of normal squamous epithelium served as controls. The expression of COX-2, Ki-67, cyclin A and p27 was examined by immunohistochemistry. The Pearson test was used to analyze the relationship between groups.

Results: The protein level of COX-2, Ki-67 and cyclin A was significantly higher in ESCC than in normal squamous epithelium (74.7 ± 61.2 vs 30.2 ± 43.4, 64.0 ± 51.6 vs 11.6 ± 2.3, 44.2 ± 32.2 vs 11.7 ± 5.0, respectively, all P < 0.01). In contrast, the protein level of p27 was significantly lower in ESCC than in normal squamous epithelium (182.0 ± 69.0 vs 266.4 ± 28.0, P < 0.01). In ESCC, COX-2 expression was correlated with T stage, the score of T1-T2 stage was lower than that of T3-T4 stage (55.0 ± 42.3 vs 83.0 ± 66.5, P < 0.05), and Ki-67, cyclin A and p27 expressions were correlated with the tumor differentiation (43.8 ± 31.7 vs 98.4 ± 84.8, 32.0 ± 19.0 vs 54.1 ± 53.7, 206.2 ± 61.5 vs 123.5 ± 68.3, respectively, all P < 0.01). COX-2 expression was positively correlated to Ki-67, cyclin A and negatively correlated to p27 expression in ESCC (r = 0.270, 0.233 and -0.311, respectively, all P < 0.05).

Conclusion: The expression of COX-2 is correlated with tumor cell invasion and is closely related to the cell proliferation in patients with ESCC.

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Figures

Figure 1
Figure 1
Expression of cyclooxygenase-2 in normal esophageal mucosa and esophageal squamous cell carcinoma. Positive staining in cytoplasm in normal mucosa (A), well-differentiated squamous cell carcinoma (SCC) (B) and poorly-differentiated SCC (C). Counterstaining with hematoxylin, × 200.
Figure 2
Figure 2
Expression of Ki-67 in normal esophageal mucosa and esophageal squamous cell carcinoma. Positive nuclei staining in normal esophageal mucosa (A, × 100), well-differentiated (B, × 200) and poorly-differentiated squamous cell carcinoma (C, × 200). Counterstaining with hematoxylin.
Figure 3
Figure 3
Expression of cyclin A in normal esophageal mucosa and esophageal squamous cell carcinoma. Positive nuclei staining in normal esophageal mucosa (A, × 100), well-differentiated (B, × 200) and poorly-differentiated squamous cell carcinoma (C, × 200). Counterstaining with hematoxylin.
Figure 4
Figure 4
Expression of p27 in normal esophageal mucosa and esophageal squamous cell carcinoma. Sections stained with anti-p27 antibody showing nuclear staining (brown) in normal esophageal mucosa (A, × 100), well-differentiated (B, × 200) and poorly-differentiated squamous cell carcinoma (C, × 200). Counterstaining with hematoxylin.
See this image and copyright information in PMC

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