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. 1977 Nov;132(2):576-83.
doi: 10.1128/jb.132.2.576-583.1977.

Transformation in pneumococcus: existence and properties of a complex involving donor deoxyribonucleate single strands in eclipse

D A Morrison

Transformation in pneumococcus: existence and properties of a complex involving donor deoxyribonucleate single strands in eclipse

D A Morrison. J Bacteriol. 1977 Nov.

Abstract

Donor deoxyribonucleic acid (DNA) single strands exist in a complex during the eclipse phase in pneumococcal transformation. This eclipse complex exhibited specific physical properties distinct from those of both pure DNA single strands and native DNA. These included a lower affinity for diethylaminoethyl-cellulose and hydroxylapatite than that of single-strand DNA, faster sedimentation than the DNA chains that it contains, and a buoyant density in Cs2SO4 lower than that of native DNA. The complex was dissociated by treatments with sodium dodecyl sulfate, NaOH, guanidine-hydrochloride, chloroform, and proteinase K but was insensitive to ribonuclease.

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References

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