Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Feb;61(2):139-42.
doi: 10.1016/j.jdermsci.2010.11.017. Epub 2010 Dec 5.

Kaposi's sarcoma and human dermal microvascular endothelial cells infected with Kaposi's sarcoma-associated herpesvirus express CCL21

Makoto Sugaya, Stacy Reed, Patrick P Rose, Stacy de la Motte, Camilo M Raggo, Stephen E Kurtz, Ashlee V Moses, Klaus Früh, Andrew Blauvelt

Kaposi's sarcoma and human dermal microvascular endothelial cells infected with Kaposi's sarcoma-associated herpesvirus express CCL21

Makoto Sugaya et al. J Dermatol Sci. 2011 Feb.
No abstract available

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
KS tumors contain CCL21+ spindle cells and CCR7+ cells (x 80). (a) CCL21+ dermal lymphatic endothelial cells (in brown) in normal skin. (b) Serial section of a KS specimen (also shown in c) incubated with no primary antibody showing no background staining. (c, d) Sections taken from two separate patients with KS showing spindle cells within dermal tumors staining for CCL21 (in brown). (e) CCR7+ positive cells in normal human spleen (in brown). (f) Serial section of a KS specimen (also shown in g) incubated with no primary antibody showing no background staining. (g, h) Sections taken from two separate patients with KS showing collections of infiltrating cells within dermal tumors staining for CCR7 (in brown).
Fig. 2
Fig. 2
KSHV infection of immortalized HDMEC induces CCL21 mRNA and protein expression and phosphorylation of Erk1/2. On four separate occasions, immortalized HDMEC were infected with KSHV or inactivated KSHV (i.e., mock-infection) and both (a) KSHV LANA and (b) CCL21 mRNA expression were found to be markedly up-regulated compared to background signals using quantitative real-time RT-PCR. (c) CCL21 protein was also enhanced in KSHV-infected endothelial cells compared to mock-infected cells by Western blot analysis. GAPDH was used as a loading control. (d) KSHV infection of immortalized HDMEC up-regulates phosphorylation of Erk1/2, which is a key pathway for expression of CCL21 in these cells.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Boshoff C, Schulz TF, Kennedy MM, Graham AK, Fisher C, Thomas A, et al. Kaposi’s sarcoma-associated herpesvirus infects endothelial and spindle cells. Nat Med. 1995;1:1274–8. - PubMed
    1. Hong YK, Foreman K, Shin JW, Hirakawa S, Curry CL, Sage DR, Libermann T, et al. Lymphatic reprogramming of blood vascular endothelium by Kaposi sarcoma-associated herpesvirus. Nat Genet. 2004;36:683–5. - PubMed
    1. Yoshida R, Nagira M, Kitaura M, Imagawa N, Imai T, Yoshie O. Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7. J Biol Chem. 1998;273:7118–22. - PubMed
    1. Debes GF, Arnold CN, Young AJ, Krautwald S, Lipp M, Hay JB, et al. Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues. Nat Immunol. 2005;6:889–94. - PMC - PubMed
    1. Moses AV, Fish KN, Ruhl R, Smith PP, Strussenberg JG, Zhu L, et al. Long-term infection and transformation of dermal microvascular endothelial cells by human herpesvirus 8. J Virol. 1999;73:6892–902. - PMC - PubMed

Publication types