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. 2011 Feb;61(2):139-42.
doi: 10.1016/j.jdermsci.2010.11.017. Epub 2010 Dec 5.

Kaposi's sarcoma and human dermal microvascular endothelial cells infected with Kaposi's sarcoma-associated herpesvirus express CCL21

Makoto Sugaya, Stacy Reed, Patrick P Rose, Stacy de la Motte, Camilo M Raggo, Stephen E Kurtz, Ashlee V Moses, Klaus Früh, Andrew Blauvelt

Kaposi's sarcoma and human dermal microvascular endothelial cells infected with Kaposi's sarcoma-associated herpesvirus express CCL21

Makoto Sugaya et al. J Dermatol Sci. 2011 Feb.
No abstract available

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Figures

Fig. 1
Fig. 1
KS tumors contain CCL21+ spindle cells and CCR7+ cells (x 80). (a) CCL21+ dermal lymphatic endothelial cells (in brown) in normal skin. (b) Serial section of a KS specimen (also shown in c) incubated with no primary antibody showing no background staining. (c, d) Sections taken from two separate patients with KS showing spindle cells within dermal tumors staining for CCL21 (in brown). (e) CCR7+ positive cells in normal human spleen (in brown). (f) Serial section of a KS specimen (also shown in g) incubated with no primary antibody showing no background staining. (g, h) Sections taken from two separate patients with KS showing collections of infiltrating cells within dermal tumors staining for CCR7 (in brown).
Fig. 2
Fig. 2
KSHV infection of immortalized HDMEC induces CCL21 mRNA and protein expression and phosphorylation of Erk1/2. On four separate occasions, immortalized HDMEC were infected with KSHV or inactivated KSHV (i.e., mock-infection) and both (a) KSHV LANA and (b) CCL21 mRNA expression were found to be markedly up-regulated compared to background signals using quantitative real-time RT-PCR. (c) CCL21 protein was also enhanced in KSHV-infected endothelial cells compared to mock-infected cells by Western blot analysis. GAPDH was used as a loading control. (d) KSHV infection of immortalized HDMEC up-regulates phosphorylation of Erk1/2, which is a key pathway for expression of CCL21 in these cells.
See this image and copyright information in PMC

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