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Comparative Study
. 2011 Mar;49(3):1097-100.
doi: 10.1128/JCM.02457-10. Epub 2010 Dec 22.

Diagnostic performance of filter paper lesion impression PCR for secondarily infected ulcers and nonulcerative lesions caused by cutaneous leishmaniasis

Andrea K Boggild  1 , Ana Pilar Ramos, Braulio Mark Valencia, Nicolas Veland, Flor Calderon, Jorge Arevalo, Donald E Low, Alejandro Llanos-Cuentas
Affiliations
Comparative Study

Diagnostic performance of filter paper lesion impression PCR for secondarily infected ulcers and nonulcerative lesions caused by cutaneous leishmaniasis

Andrea K Boggild et al. J Clin Microbiol. 2011 Mar.

Abstract

We compared traditional cutaneous leishmaniasis diagnostic methods to filter paper lesion impression (FPLI) PCR for secondarily infected ulcers and nonulcerative lesions. The sensitivity and specificity of FPLI PCR for secondarily infected lesions (n = 8) were 100%. In primarily nonulcerative lesions (n = 15), the sensitivity of FPLI PCR was inferior to that of pooled-invasive-specimen PCR (72.7% versus 100%) (P = 0.10). FPLI PCR is sensitive, specific, and unlike invasive procedures, can be used in secondarily infected ulcers. Invasive specimen collection is superior in nonulcerative lesions.

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